Ettan dige system user manual

DeCyder 2D Software User Manual AB 9 Fig Scheme showing the workflow for Ettan DIGE system The benefits offered by Ettan DIGE system are: • Accurate quantitation and statistica l analysis of protein abundance changes • High sensitivity and wide dynamic range (5 orders of magnitude) • Minimization of system (gel-to-gel) variation. University of Alberta. Protocol manual: 2D Electrophoresis, Principles and Methods, GE Healthcare. DeCyder 2D software version user manual, GE Healthcare. Ettan DIGE System, User Manual, GE Healthcare.

2-D DIGE is a platform for the analysis of differential protein expression. When you want to detect and analyze differences or changes in protein levels/expression between complex protein samples, 2-D DIGE combines confidence with reproducibility to give dependable results. The Ettan™ DIGE System consists of. CyDye DIGE Fluor minimal dye to 50 µg protein followed by incubation on ice in the dark for 30 min. The reaction was quenched by the addition of 10 mM lysine (1 µl per pmol CyDye DIGE Fluor minimal dye) followed by incubation on ice for a further 10 min (see Ettan DIGE User Manual for detailed protocols). Table 2. DeCyder 2D Software User Manual AB 9 Fig Scheme showing the workflow for Ettan DIGE system The benefits offered by Ettan DIGE system are: • Accurate quantitation and statistica l analysis of protein abundance changes • High sensitivity and wide dynamic range (5 orders of magnitude) • Minimization of system (gel-to-gel) variation.

Please sign in to view account pricing and product availability. Sign In. Don't have a profile?Register · Basket0. A key element in DIGE is the use of pooled internal standard Equal amounts of each of the The Ettan™ system comprises CyDye™ DIGE Fluor minimal dyes. These dyes are patent protected and do not infringe the Ettan® DIGE patent a detailled decription of an analysis in the Getting Started guide for DIGE.